Association of Polymorphisms in DAZL Gene with Male Infertility
Omar F. Khabour *
Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid 22110, Jordan.
Akram M. Al-azzam
King Hussein Medical Center, Amman, Jordan.
Almuthana A. Alfaouri
King Hussein Medical Center, Amman, Jordan.
Faheem Zayed
Department of Obstetrics and Gynaecology , Jordan University of Science and Technology, Irbid 22110, Jordan.
*Author to whom correspondence should be addressed.
Abstract
Objectives: To investigate the association between A260G (Thr12Ala) and A386G (Thr54Ala) single nucleotide polymorphisms (SNPs) in deleted in azoospermia-like gene (DAZL) and infertility in Jordanian males.
Methods: Infertile 170 patients with azoospermia or oligozoospermia and 176 fertile subjects were recruited in the study. DAZL SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP).
Results: The data showed that the A260G SNP is common in the Jordanian population with frequency of 10.3% for 260G mutant allele. However, the A386G SNP is absent in the studied population. No significant association was found between the examined SNPs in DAZL gene and men infertility.
Conclusion: The A260G and A386G polymorphisms of DAZL seem to play no role in men infertility in Jordanian population.
Keywords: Deleted in azoospermia-like gene, single nucleotide polymorphism, Jordan, infertility, male