Effect of 67Zn-Nanoparticles on Leukemic Cells and Normal Lymphocytes
Marina A. Orlova *
Department of Chemistry, M. V. Lomonosov Moscow State University, 1/3, Leninskie Gory, Moscow, 119899, Russian Federation
Elena Yu. Osipova
Federal Research Center of Pediatric Hematology, Oncology and Immunology FSI, bd.2, 117, Lenin Ave., Moscow, 117997, Russian Federation
Sergey A. Roumiantsev
Federal Research Center of Pediatric Hematology, Oncology and Immunology FSI, bd.2, 117, Lenin Ave., Moscow, 117997, Russian Federation.
*Author to whom correspondence should be addressed.
Abstract
Porphyrin-fulleren-based nanoparticles (NP), containing magnetic isotopes 25Mg, 67Zn and zinc of natural isotope composition (Zntotal-NP), have been tested on leukemic cells of patients with T-ALL, B-ALL, AML and lymphocytes of healthy donors. Reliable differences in action of magnetic and non-magnetic zinc isotopes for some types of cells were obtained. Magnetic magnesium isotopes and pure nanoparticles of porphyrinfulleren did not demonstrate any effects. 67Zn-NP induced high cytotoxicity in cells of acute B-lymphoblastic leukemia with LD50 almost three times lower, than those for healthy donors, and 4 times lower in comparison with Zntotal-NP. Also evaluation of apoptosis process in granulocytes of healthy donors in the case of the preparates were performed by method of flow cytometry.
Keywords: Fullerene-based nanoparticles, Lsotopy, 67Zn, 25Mg, apoptosis, MTT-method, flow cytometry