Biological Characteristics of Mesenchymal Stem Cells during Ex Vivo Expansion
E. Y. Osipova *
Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology, 117/2 Leninsky Prosp., 117420 Moscow, Russian Federation.
T. V. Shamanskaya
Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology, 117/2 Leninsky Prosp., 117420 Moscow, Russian Federation.
O. A. Kurakina
Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology, 117/2 Leninsky Prosp., 117420 Moscow, Russian Federation.
V. A. Nikitina
Research Centre for Medical Genetics, Russian Academy of Medical Sciences, 1 Moskvorech’e str., 115478, Moscow, Russian Federation.
B. B. Purbueva
Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology, 117/2 Leninsky Prosp., 117420 Moscow, Russian Federation.
A. Y. Ustugov
Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology, 117/2 Leninsky Prosp., 117420 Moscow, Russian Federation.
D. Y. Kachanov
Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology, 117/2 Leninsky Prosp., 117420 Moscow, Russian Federation.
E. V. Skorobogatova
Russian Children’s Hospital, 117/2 Leninsky Prosp., 117420 Moscow, Russian Federation.
Z. M. Dishlevaya
Russian Children’s Hospital, 117/2 Leninsky Prosp., 117420 Moscow, Russian Federation.
S. A. Roumiantsev
Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology, 117/2 Leninsky Prosp., 117420 Moscow, Russian Federation.
*Author to whom correspondence should be addressed.
Abstract
Mesenchymal stem cells (MSC) have been widely used in different areas of medicine because of their immunosupressive properties and influence on regeneration of the damaged tissues. The objective of this study was to examine the kinetics of growth (MSC from 4-3 passages had significantly higher proliferative activity compared with cultures of 10-12 passages), changes in immunophenotypic characteristics of human MSC in early (3-4) and late (10-12) passages when cultured in vitro as well as study of genetic stability. In the analyzed cultures of MSC was found normal karyotype (46, XY or 46, XX). In most of cultures studied MSC karyotype and the level of aneuploidy remained unchanged even after prolonged culturing.
The author's experience of ex vivo expansion of MSC using both standard medium DMEM with the supplementation of fetal bovine serum (FBS) and serum-free medium MesenCult MSC Basal Medium (Human) (StemCell technologies Inc.) with addition of Mesenchymal Stem Cell Stimulatory Supplements (Human) is presented. The influence of the type of medium on the growth and immunophenotype of MSC is analyzed. The study found that when cultured in serum-free medium proliferative potential of MSCs was lower.
Keywords: Mesenchymal stem cells, phenotypic profile, genetic stability, expansion ex vivo, FCS-free culture