Immunological Profiles of Mice Protected from Chlamydia-induced Infertility by Anti-caspase Treatment
C. E. Ukwade
Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria
O. A. T. Ebuehi *
Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria
J. U Igietseme
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA
S. Ouburg
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA
J. A. Land
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA
Y. Omosun
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA
K. Joseph
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA
J. Partin
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA
Q. He
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA.
F. O. Eko
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA
D. Ellerson
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA
C. Bandea
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA
S. A. Morre
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA
G. Zhong
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA
C. M. Black
Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia, USA
*Author to whom correspondence should be addressed.
Abstract
The study is to investigate the effect of anti-caspase treatment on anti-chlamydia immune response in mice. Both the humoral and aspects of cell-mediated immune response against Chlamydia trachomatis were studied. Antibody response was measured using the ELISA technique to identify all common isotypes, and cytokine response was measured using the PCR technique. The antibody levels (IgG, IgG1, IgG2a and IgA) in Z-VAD-FMK treated group were significantly higher than non-treated group. ELISA results [showed a significantly higher amount of antibodies (IgG, IgG1, Ig G2a and IgA)] were produced in the mice that were pre-treated with Z-VAD-FMK before infection with Chlamydia trachomatis compared to mice post treated with Z-VAD-FMK after Chlamydia trachomatis infection. Data of the study indicate that the caspase inhibitor, Z-VAD-FMK did not negatively affect humoral and T cell mediated immune responses against C. trachomatis in mice.
Keywords: Chlamydia trachomatis, anti-caspase treatment, immune response, infertility, mice