Comparative Evaluation of Apical Sealing Ability Following Canal Preparation with Different Instrumentation Techniques

Pavithra Gopal *

Department of Conservative Dentistry and Endodontics, DAPM RV Dental College, India.

H Murali Rao

Department of Conservative Dentistry and Endodontics, DAPM RV Dental College, India.

B. S. Keshava Prasad

Department of Conservative Dentistry and Endodontics, DAPM RV Dental College, India.

Harrini K

Department of Conservative Dentistry and Endodontics, DAPM RV Dental College, India.

*Author to whom correspondence should be addressed.


Abstract

Background: Success in root canal therapy is dependent on effective cleaning, shaping, and establishment of a fluid tight apical seal. Instrumentation techniques significantly influence canal morphology and the quality of obturation.

Aim: To evaluate and compare the apical sealing ability of obturations after canal preparation with different instrumentation techniques.

Materials and Methods: In this in vitro study -Forty extracted mandibular premolars with single canals were standardized to 14 mm root length and randomly assigned to four groups (n=10 each). Group 1 – Step back technique with stainless steel hand files; Group 2 – Modified step back technique with hand files; Group 3 – Crown down technique with greater taper manual NiTi files; Group 4 – Crown down technique with greater taper rotary NiTi files. Following cleaning and shaping, all canals were obturated with gutta-percha and bioceramic sealer by lateral condensation. Coronal access was sealed with glass ionomer cement, and samples were stored at 37°C for 24 hours. Root surfaces were coated with varnish except for the apical 3 mm and immersed in 1% methylene blue dye for 72 hours. Longitudinal sections were examined under stereomicroscope at 30× magnification, and dye penetration was measured using ImageJ software. Data were statistically analyzed with ANOVA and Tukey’s test.

Results: Mean dye penetration was highest in Group 1 (1.37 ± 0.24 mm), followed by Group 2 (1.06 ± 0.32 mm), Group 3 (0.78 ± 0.18 mm), and lowest in Group 4 (0.62 ± 0.16 mm). Significant differences were observed between step back and crown down groups (p < 0.05). No significant difference was noted between manual and rotary crown down groups.

Conclusion: Crown down instrumentation using greater taper NiTi files provided superior apical sealing compared to step back methods. Incorporating coronal pre-flaring and progressive taper preparation enhances obturation quality. Both manual and rotary crown down techniques are effective, though rotary instruments improve efficiency.

Keywords: Apical seal, crown down technique, step back technique, nickel–titanium files, microleakage, endodontics


How to Cite

Gopal, Pavithra, H Murali Rao, B. S. Keshava Prasad, and Harrini K. 2025. “Comparative Evaluation of Apical Sealing Ability Following Canal Preparation With Different Instrumentation Techniques”. Journal of Advances in Medicine and Medical Research 37 (11):122-31. https://doi.org/10.9734/jammr/2025/v37i115972.

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