Efficacy of Chitosan in Reducing Tooth Sensitivity Induced by in-Office Bleaching: A Randomized, Double-blind, Controlled Clinical Trial

Wesley Fernando Ferrari

Universidade Paranaense (UNIPAR), Umuarama, Brazil.

Nubia Inocencya Pavesi Pini

Ingá University Center (UNINGÁ), State University of Maringá (UEM), Maringá, Brazil.

Maria Ritha Veiga Colognese *

State University of West Paraná (UNIOESTE), Cascavel, Brazil.

Giovani Ceron Hartmann

Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, Brazil.

Mauro Carlos Agner Busato

State University of West Paraná (UNIOESTE), Cascavel, Brazil.

Márcio José Mendonça

State University of West Paraná (UNIOESTE), Cascavel, Brazil.

Veridiana Camilotti

State University of West Paraná (UNIOESTE), Cascavel, Brazil.

*Author to whom correspondence should be addressed.


Abstract

Aims: To evaluate the effect of incorporating chitosan microparticles into a commercial desensitizing agent on tooth sensitivity during and after in-office bleaching procedures.

Study Design: Randomized, controlled, double-blind clinical trial.

Place and Duration of Study: Department of Restorative Dentistry of State University of West Paraná (UNIOESTE), between June and December 2017.

Methodology: Forty-two patients were selected and randomly assigned to two groups (n = 21 each). The DKF group received only a commercial desensitizing agent, while the DKF + CHI group received the same agent combined with chitosan microparticles. In both groups, 35% hydrogen peroxide was used for in-office bleaching on the upper arch. The desensitizing gel was applied for 10 minutes prior to bleaching. Tooth sensitivity was self-reported by patients using a visual analog scale at 5-minute intervals during bleaching (up to 45 minutes) and at 1, 24, and 48 hours post-procedure. Color change was evaluated at baseline and after 7 days. The Mann–Whitney U test was used for statistical analysis (P < 0.05).

Results: The DKF + CHI group reported significantly lower sensitivity levels than the DKF group at 35, 40, and 45 minutes during the procedure, and at 60 minutes post-treatment (p < 0.05). The probability of pain occurrence was markedly reduced in the group treated with chitosan, supporting its potential effectiveness as a desensitizing adjunct in clinical bleaching protocols. No statistically significant differences were observed between the groups regarding bleaching effectiveness.

Conclusion: The incorporation of chitosan microparticles into the desensitizing agent effectively reduces tooth sensitivity associated with in-office bleaching procedures, without compromising the bleaching outcome.

Keywords: Bleaching, chitosan, dentin sensitivity, enamel, tooth whitening


How to Cite

Ferrari, Wesley Fernando, Nubia Inocencya Pavesi Pini, Maria Ritha Veiga Colognese, Giovani Ceron Hartmann, Mauro Carlos Agner Busato, Márcio José Mendonça, and Veridiana Camilotti. 2025. “Efficacy of Chitosan in Reducing Tooth Sensitivity Induced by in-Office Bleaching: A Randomized, Double-Blind, Controlled Clinical Trial”. Journal of Advances in Medicine and Medical Research 37 (5):114-25. https://doi.org/10.9734/jammr/2025/v37i55821.

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