Effects of α-Humulene and its Nanoparticles in Experimental Model of Alzheimer's Disease: Behavioral Analysis and Anti-Inflammatory Activity
Maria Elvira Ribeiro Cordeiro *
University of Brasilia, DF, Brazil.
Luana Rodrigues Vasconcelos
Mid-West State University, PR, Brazil.
Heron Bittencourt
Mid-West State University, PR, Brazil.
Flávio Klinpovous Kerppers
Mid-West State University, PR, Brazil.
Andressa Panegalli Hosni
Mid-West State University, PR, Brazil.
Ivo Ilvan Kerppers
Department of Physiotherapy, Mid-West State University, PR, Brazil.
Rubiana Mara Mainardes
Pharmacy School, Mid-West State University, Guarapuava, PR, Brazil.
Christiane Schineider Machado Kolc
Mid-West State University, PR, Brazil.
Ana Carolina Dorigoni Bini
Mid-West State University, PR, Brazil.
Patricia Pacheco Tyski Suckow
Mid-West State University, PR, Brazil.
Eliane Gonçalves de Jesus Fonseca
Mid-West State University, PR, Brazil.
Angela Dubiela Julik
Mid-West State University, PR, Brazil.
*Author to whom correspondence should be addressed.
Abstract
The aim was to analyze the effects of α-humulene in experimental models of Alzheimer’s disease (AD). A sample composed of 33 rats, divided into the NC group: 3 animals, for analysis of normal tissue and behavior, PC: 10 animals submitted to induction of AD by application of β-amyloid, without treatment, HUM: 10 animals submitted to induction of AD and treated with α-humulene, and NHUM: 10 animals submitted to induction of AD and treated with α-humulene nanoparticles. Treatment was performed once daily for 14 days at a dose of 6.5µg, orally. The animals were submitted to behavioral tests of spatial memory and aversive memory, the cytokines TNF, IL-10, IL-2 and IL-4 were analyzed by flow cytometry. The treated animals obtained superior cognitive performance and expressed lower levels of inflammatory markers, with better results in the group with better results in the NHUM.
Keywords: α-humulene, Alzheimer's, nanoparticles, cytokine, neuroinflammation