Efficacy of Ozonized Sunflower Oil with Tea Tree Oil as Desensitizing Agents in Dental Bleaching: Randomized and Double-Blind Clinical Trial

Luiza Maria Schneider

Western State University of Paraná - Dental School, Brazil.

Poliana Maria de Faveri Cardoso *

Western State University of Paraná - Dental School, Brazil.

Julio Katuhide Ueda

Western State University of Paraná - Dental School, Brazil.

Márcio José Mendonça

Western State University of Paraná - Dental School, Brazil.

Veridiana Camilotti

Western State University of Paraná - Dental School, Brazil.

*Author to whom correspondence should be addressed.


Background: Dental bleaching is one of the most commonly prescribed procedures in aesthetic dentistry due to its effectiveness and minimally invasive nature. However, a recurrent and challenging side effect is dentin hypersensitivity after the procedure.

Aim: This study aimed to evaluate whether the use of ozonized oil combined with tea tree oil is effective in controlling post-bleaching dentin sensitivity.

Methods: A randomized, double-blind, split-mouth clinical trial was conducted. Participants were divided into two experimental groups (n=29) according to the desensitization protocol: control group: potassium nitrate and sodium fluoride; test group: ozonized sunflower oil combined with tea tree oil. Both groups were applied before dental bleaching with 35% hydrogen peroxide. The obtained data were subjected to statistical analysis using a non-parametric Friedman repeated measures ANOVA test (p < 0.05), followed by the Durbin-Conover post hoc test (p < 0.05).

Results: In the assessment of sensitivity level, there were no statistically significant differences between the test group and the control group. A higher level of sensitivity was observed for the lower arch after one hour of bleaching agent removal in both groups, and the color change was statistically equal between the two groups.

Conclusion: The study concluded that the test group paste was as effective in reducing dental sensitivity caused by dental bleaching as the control group. Additionally, neither of the groups affected the bleaching capacity of hydrogen peroxide.

Keywords: Ozone, dentin sensitivity, tooth bleaching, ozonized sunflower oil, tea tree oil

How to Cite

Schneider , Luiza Maria, Poliana Maria de Faveri Cardoso, Julio Katuhide Ueda, Márcio José Mendonça, and Veridiana Camilotti. 2023. “Efficacy of Ozonized Sunflower Oil With Tea Tree Oil As Desensitizing Agents in Dental Bleaching: Randomized and Double-Blind Clinical Trial”. Journal of Advances in Medicine and Medical Research 35 (21):18-27. https://doi.org/10.9734/jammr/2023/v35i215206.


Download data is not yet available.


Gasmi Benahmed A, Gasmi A, Menzel A, Hrynovets I, Chirumbolo S, Shanaida M, Lysiuk R, Shanaida Y, Dadar M, Bjørklund G, A review on natural teeth whitening, J. Oral Biosci. 2022;64:49–58.


Rodríguez-Martínez J, Valiente M, Sánchez-Martín MJ. Tooth whitening: From the established treatments to novel approaches to prevent side effects. J. Esthet. Restor. Dent. 2019;31:431–440.


Alkahtani R, Stone S, German M, Waterhouse P. A review on dental whitening, J. Dent. 2020;100:103423.


Krishnakumar K, Tandale A, Mehta V, Khade S, Talreja T, Aidasani G, Arya A. Post-operative sensitivity and color change due to in-office bleaching with the prior use of different desensitizing agents: A systematic review. Cureus. 2022;14. Available:https://doi.org/10.7759/cureus.24028

West NX, Lussi A, Seong J, Hellwig E, Dentin hypersensitivity: Pain mechanisms and aetiology of exposed cervical dentin, Clin. Oral Investig. 2013;17:9–19. Available:https://doi.org/10.1007/s00784-012-0887-x

Dietrich L, de Assis Costa MDM, Blumenberg C, Nascimento GG, Paranhos LR, da Silva GR. A meta-analysis of ozone effect on tooth bleaching. Sci. Rep. 2021; 11:1–12. Available:https://doi.org/10.1038/s41598-021-92733-8

Sen S, Sen S. Ozone therapy a new vista in dentistry: Integrated review, Med. Gas Res. 2020;10:189–192.


Taalab MR, Mahmoud SA, Moslemany RME, Abdelaziz DM, Intrapocket application of tea tree oil gel in the treatment of stage 2 periodontitis, BMC Oral Health. 2021;211–10. Available:https://doi.org/10.1186/s12903-021-01588-y

Al-Omiri MK, Al Nazeh AA, Kielbassa AM, Lynch E. Randomized controlled clinical trial on bleaching sensitivity and whitening efficacy of hydrogen peroxide versus combinations of hydrogen peroxide and ozone. Sci. Rep. 2018;8:1–10. Available:https://doi.org/10.1038/s41598-018-20878-0

Teixeira N, Webber MBF, Nassar CA, Camilotti V, Mendonça MJ, Sinhoreti MAC, Influence of different composites and polishing techniques on periodontal tissues near noncarious cervical lesions: a controlled, randomized, blinded clinical trial. Eur. J. Dent. 2019;13:635–641. Available:https://doi.org/10.1055/s-0039-3401900

Wiggers IF, Mendonça MJ, Vanolli R, Campos F, Ueda JK, Colognese MRV, Camilotti V. Ozonated oil as a desensitizing protocol in external dental bleaching : A clinical , randomized and double-blind study. J. Adv. Med. Med. Res. 2023;35:10–20. Available:https://doi.org/10.9734/JAMMR/2023/v35i155064

Tay LY, Kose C, Loguercio AD, Reis A. Assessing the effect of a desensitizing agent used before in-office tooth bleaching. Joural Am. Dent. Assoc. 2009;140:1245–1251.

Godoy CEM, Consani S, Guimarães ATB, Laurindo BM, Mendonça MJ, Camilotti V. Effect of two desensitizing agents applied previous to in-office bleaching on the degree of whitening and dentin sensitivity: A randomized, controlled, double-blind clinical trial. Am. J. Dent. 2021;34:70–74.

Rezende M, da Silva KL, Miguel TC, Farago PV, Loguercio AD, Martins LD, Reis A. Prior application of 10% potassium nitrate to reduce postbleaching sensitivity: A randomized triple-blind clinical trial. J. Evid. Based. Dent. Pract. 2020;20:101406. Available:https://doi.org/10.1016/j.jebdp.2020.101406

Suh Y, Patel S, Kaitlyn R, Gandhi J, Joshi G, Smith N, Khan S. Clinical utility of ozone therapy in dental and oral medicine. Med. Gas Res. 2019;9:163–167. Available:https://doi.org/10.4103/2045-9912.266997

Veena HR, Afigith Mathew C, Daniel RA, Shubha P, Sreeparvathy R, Pradhan N. An in vitro analysis of the effect of adjunctive use of ozonated oil with a desensitizing agent on dentinal tubule occlusion. J. Oral Biol. Craniofacial Res. 2020;10:727–732. Available:https://doi.org/10.1016/j.jobcr.2020.10.001

Nogales CG, Ferreira MB, Montemor AF, de A. Rodrigues MF, Lage-Marques JL, Antoniazzi JH. Ozone therapy as an adjuvant for endondontic protocols: Microbiological – Ex vivo study and citotoxicity analyses. J. Appl. Oral Sci. 2016;24:607–613. Available:https://doi.org/10.1590/1678-775720160029

Tricarico G, Rodrigues Orlandin J, Rocchetti V, Ambrosio CE, Travagli V. A critical evaluation of the use of ozone and its derivatives in dentistry. Eur. Rev. Med. Pharmacol. Sci. 2020;24:9071–9093.


Carson CF, Hammer KA, Riley TV. Melaleuca alternifolia (tea tree) oil: A review of antimicrobial and other medicinal properties. Clin. Microbiol. Rev. 2006;19: 50–62. Available:https://doi.org/10.1128/CMR.19.1.50-62.2006

Stea S, Beraudi A, De Pasquale D. Essential oils for complementary treatment of surgical patients: State of the art, Evidence-Based Complement. Altern. Med.; 2014. Available:https://doi.org/10.1155/2014/726341