Role of Some Apoptotic Proteins in Pathogenesis of Juvenile Systemic Lupus Erytheromatosis and Lupus Nephritis
Anne Albir Aziz Hanna
Pediatrics Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Hend Hassan Abdelnabi
Pediatrics Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Naglaa Mohamed Kholoussi
Immunogenetics Department, Human Genetics Division, National Research Center, Egypt.
Nagy Mohamed Abu El Hana
Pediatrics Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Background: Apoptosis is increased in Systemic Lupus Erytheromatosis (SLE) patients with defective clearance of apoptotic cells, which lead to a pathological accumulation of abnormal cell debris and provide an autoimmune reaction. The aim of this work was to evaluate apoptosis and CD4/CD8 ratio in juvenile SLE patients and their relation to disease activity and lupus nephritis (LN).
Methods: This case-control study included 50 patients with jSLE divided into two groups according to SLEDAI score and twenty-five healthy children of matched age and sex as controls. All cases were subjected to detection of apoptosis, in the early stage through investigation of Annexin V and CD4/CD8 ratio through CD4 and CD8 T cells monoclonal antibody using the BD Accuri™ C6 Flow Cytometer Instrument.
Results: early apoptosis has statistically significant increase in the jSLE patients compared to controls with positive correlation to disease activity and no correlation to LN. (active group 20.77± 7.88, inactive group13± 6.8 and controls3.53± 1.26). CD4/CD8 ratio has significant decrease in patients compared to controls with inversed correlation to disease activity and no correlation to LN. (active group1.28 ± 0.51, inactive group1.6 ± 00.6 and controls 2.1 ± 0.6.
Conclusions: Apoptosis is high in jSLE patients with low CD4/CD8 ratio which related to disease activity, but not related to LN.
Keywords: Apoptotic Proteins, pathogenesis, juvenile systemic lupus erytheromatosis, lupus nephritis