The Polymorphism of Regulatory Region of NOS3 Gene (rs2070744, Genotype CC) Protect Patients from Chronic Migraine
Eugene Klimov *
Department of Genetics, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia and University Diagnostic Laboratory, Moscow, Russia.
Natalia Kondratieva
Department of Genetics, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
Kirill Skorobogatykh
University Headache Clinic, Moscow, Russia.
Julia Azimova
University Headache Clinic, Moscow, Russia.
Alexey Sergeev
University Headache Clinic, Moscow, Russia and Department of Neurology, Research Center of the I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
Zarema Kokaeva
Department of Genetics, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
Elena Naumova
Department of Genetics, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia and University Diagnostic Laboratory, Moscow, Russia.
Andrey Rachin
University Headache Clinic, Moscow, Russia and Department of Neurology, Federal State-Funded Institution, Russian Scientific Center for Medical Rehabilitation and Balneology, Moscow, Russia.
Gyuzyal Tabeeva
University Headache Clinic, Moscow, Russia and Department of Neurology, Research Center of the I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
*Author to whom correspondence should be addressed.
Abstract
Aims: There is evidence that endothelial nitric oxide synthase has a role in migraine pathophysiology. In our research, the role of SNP rs2070744 (c.-813C>T) in promoter region of NOS3 gene in the episodic and chronic forms of migraine is considered.
Place and Duration of Study: University Headache Clinic between June 2012 and November 2014 and Department of Genetics, Faculty of Biology of Lomonosov Moscow State University between October 2013 and March 2016.
Methodology: The study included 138 patients with migraine (44 with chronic and 96 with episodic migraine). The control group included 348 unexamined subjects. Genotypes were determined using real-time PCR with allelic discrimination test. Statistical processing was performed using Fisher test and Pearson's chi-squared test.
Results: Our study evaluated the link of CC genotype of rs2070744 with migraine (Fisher’s p=0.026) and episodic migraine (Fisher’s p=0.022).
Conclusion: Genotype CC of SNP rs2070744 in the regulatory region of NOS3 gene is more specific for episodic migraine and may prevents the chronification of migraine.
Keywords: NOS3, rs2070744, migraine, chronic migraine, episodic migraine.