Baseline Tryptase Levels Correlate with Baseline Basophil Levels in Chronic Myeloid Leukemia-Chronic Phase
Anisha Mathew
Department of Biochemistry, Lady Hardinge Medical College, Delhi, India.
Manisha Naithani *
Department of Biochemistry, All India Institute of Medical Sciences, Rishikesh, India.
Sarama Saha
Department of Biochemistry, All India Institute of Medical Sciences, Rishikesh, India.
Rituparna Chetia
Department of Medical Oncology Hematology, All India Institute of Medical Sciences, Rishikesh, India.
Uttam Kumar Nath
Department of Medical Oncology Hematology, All India Institute of Medical Sciences, Rishikesh, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: To study whether there is any correlation between baseline blood basophil count and serum tryptase levels in newly diagnosed chronic phase chronic myeloid leukemia (CML-CP) patients.
Settings and Design: 40 newly diagnosed CML-CP patients were enrolled from Medical Oncology Hematology OPD based on their baseline BCR-ABL status (done in department of Biochemistry).
Methods and Materials: Serum tryptase level was measured using Sandwich ELISA and peripheral blood basophil count was estimated using automated cell counter & peripheral blood film examination. BCR-ABL quantification was done using real time PCR after conversion of RNA (extracted from whole blood) to cDNA.
Statistical Analysis Used: SPSS Version 23.
Results: Baseline peripheral blood basophil levels showed a significant correlation with baseline serum tryptase levels (p<0.01) and tryptase level also correlated with EUTOS score, which has basophil count as one of the parameters. This may signify that serum tryptase levels can be a surrogate marker of the basophil compartment in CML-CP.
Conclusions: Based on findings of the present study and other studies available in literature, serum tryptase can be utilised as a surrogate marker of the basophil compartment in CML-CP.
Keywords: Basophil marker, serum tryptase, novel biomarker, serine protease.