Hyphal Formation is Enhanced by 121°C Heat Inactivation of Serum Added to Culture Media
Hong He *
Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, 395 Yan’an Road, Hangzhou, 310006, China and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, China.
Xueting Wang
Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, 395 Yan’an Road, Hangzhou, 310006, China and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, China.
Congcong Li
Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, 395 Yan’an Road, Hangzhou, 310006, China and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, China.
Xiaotong Deng
Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, 395 Yan’an Road, Hangzhou, 310006, China and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, China.
Yan Fan
Hangzhou Stomatology Hospital, Pinghai Road, Hangzhou, 310000, China.
Jianxin Han
Department of Food Science and Nutrition, School of Biosystems Engineering and Food Science, Zhejiang University, 310058, China.
*Author to whom correspondence should be addressed.
Abstract
Background: A novel method for inducing hyphae formation, was described.
Methods: Mycelia of experimental and clinical strains from 100 patients with oral lichen planus were cultured in RPMI 1640 medium with 56°C or 121°C inactivated calf sera. After these strains were cultured for 2 h to 7 days, the average hyphae formation rate was observed under light microscopy.
Results: The 121°C inactivated calf serum showed better outcomes than 56°C inactivated calf serum in terms of time and amounts of hyphae products.
Conclusions: For Candida albicans hyphae culture, the 121°C inactivated serum is superior to the 56°C inactivated serum.
Keywords: Serum inactivation, Candida albicans, hyphae, yeast, oral lichen planus.