A Clinical and Preliminary Study for Anti-angiogenesis Therapy: Endostatin, VEGF and Microvessel Density in the OSCC in Different Stages and Differentiations
Hong He
The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, China and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Stomatology, China.
Jiaqin Wang
The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, China and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Stomatology, China.
Xiaotong Deng *
The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, China and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Stomatology, China.
Danping Zhao
Second Affiliated Hospital, Zhejiang University School of Medicine, China.
*Author to whom correspondence should be addressed.
Abstract
Aims: This study was to analyze the association among ES, VEGF, Microvessel Density (MVD), clinicopathologic characteristics, angiogenesis and prognosis of OSCC.
Methods: Eight normal samples of oral epithelia and 52 Oral Squamous Cell Carcinoma (OSCC) samples were analyzed by immunohistochemical evaluation to study the expression and significance of Endostatin (ES) and Vascular Endothelial Growth Factor (VEGF) during the development of OSCC.
Results: Statistically significant differences were found as p<0.05 between VEGF expressions and clinicopathologic stages of OSCC and as p<0.01 between VEGF expressions and lymph node metastases of OSCC. And Statistically significant discrepancy was also found as p<0.05 between MVD and differentiation degrees and lymphnode metastases of OSCC, as well as p<0.01 between VEGF expressions and MVD. Additionally MVD increased gradually in accordance with the progression of the Cancer. While there was no obvious correlation between ES and VEGF, ES and MVD, as well as between ES and the development of OSCC.
Conclusion: By MVD et al evaluation, VEGF is one of the major angiogenesis factors for angiogenesis and lymphonode metastasis of oral carcinomas, as an important indicator for the development and malignancy of OSCC, while ES is of significance for anti-angiogenesis in tumor therapy.
Keywords: Endostatin, vascular endothelial growth factor, microvessel density, OSCC, immunohistochemistry.