The Sub – Acute Effects of Raw Honey on Prothrombin Time, Activated Partial Thromboplastin Time and Platelet Values in Albino Wistar Rats

Main Article Content

Akudo Chidinma Ohale
Emmanuel Ifeanyi Obeagu
Happiness Alaribe Mark
Chidindu C. Mmadu- Okoli
Chukwuebuka Brian Ugoh-Ezepue
Chibuike Ernest Ohanu
Uzoma Chinyere Okongwu
Chidinma Ijeoma Nlemadim
Ifeoma Blessing Ekeigwe
Ogochukwu Adamaka Okeke


For centuries honey has been regarded as wonderful gift of nature in which the properties of an excellent food, beneficial alike to adults and children, are combined with medicinal properties. Surprisingly, its sub-acute effect on coagulation is unknown. Hence; this present study aims at evaluating the effects of raw honey on coagulation in albino wistar rats. Thirty (30), 3-4 months old albino wistar rats both males and females were used for the study. The experimental animals were divided into five (A, B, C, D, E) groups with six rats per group. The test groups (B-E) were gavaged with graded doses (625, 1250, 2500, 5000 mg/kg body weight) respectively of the raw honey once daily for nine days. Group A served as control. Two (2) animals were bled from each group after 3, 6 and 9 days through the ocular plexus. Four (4) ml of venous blood was collected. Two (2) ml was delivered into 0.25ml trisodium citrate anticoagulant bottle for determination of Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT). The remaining two (2) ml was delivered into K3EDTA anticoagulant bottle for platelet value determination. There was no statistical significant difference (P > 0.05) recorded in all the parameters investigated among the test groups when compared with the control group on Day 3. However, group B revealed a statistical significant decrease (P < 0.05) in PT when compared with the control group on Day 6. In addition, no statistical significant difference (P>0.05) was recorded on Day 9 when all the parameters investigated among the test groups were compared with the control group. Furthermore, there was no exposure related statistical significant difference (P>0.05) in the test groups in PT and APTT in the ANOVA.  However, there was a time related significant difference (P<0.05) in platelet value of group C when Days 3, 6 and 9 were compared. It can be concluded that raw honey possesses a sub-acute coagulation stimulatory potentials which is likely to be dose and duration related.

Raw honey, prothrombin time, activated partial thromboplastin time, platelet values, albino wistar rats.

Article Details

How to Cite
Ohale, A. C., Obeagu, E. I., Mark, H. A., Okoli, C. C. M.-, Ugoh-Ezepue, C. B., Ohanu, C. E., Okongwu, U. C., Nlemadim, C. I., Ekeigwe, I. B., & Okeke, O. A. (2020). The Sub – Acute Effects of Raw Honey on Prothrombin Time, Activated Partial Thromboplastin Time and Platelet Values in Albino Wistar Rats. Journal of Advances in Medicine and Medical Research, 32(17), 68-73.
Original Research Article


Gunther RT. The Greek Herbal of Dioscorides. New York: Hafner; 1959.

Molan PC. The Antibacterial Activity of Honey. 1. The Nature of the Antibacterial Activity. Bee World. 1992;73(1):5-28.

Brandy NF, Molan PC, Harfoot CG. The Sensitivity of Dermatophytes to the Antimicrobial Activity of Maunka Honey and other honey. Pharmaceutical Sciences. 1997;2:1-3.

Select Committee on Science and Technology. Resistance to Antibiotics and other Antimicrobial Agents. London House of Lords, Report No. 1998;7.

Furie B, Furie BC. Thrombus Formation in vivo. Journal of Clinical Investigations. 2005;115(12):3355 –3362.

Dacie JV, Lewis SM. Practical Haematology 10th Edition Churchill Livingstone, Edinburgh, London. 2006;398–440.

Martos I, Ferreres F, Tomás-Barberán F. Identification of Flavonoid Markers for the Botanical Origin of Eucalyptus Honey. Journal of Agriculture and Food Chemistry. 2000;48(5):1498– 1502.

Ghleldof N, Wang X, Engeset N. Identification and Quantification of Antioxidant Components of Honey from various Floral Sources. Journal of Agriculture and Food Chemistry. 2002;59(21):5870–5877.