Haptoglobin Phenotype and Soluble Transferrin Receptor among Tuberculosis
Philemond By
Laboratoire de Biochimie, Pharmacodynamie, UFR Bosciences, Université Félix Houphouet- Boigny, Côte d’Ivoire.
H. T. Ahiboh *
Centre de Diagnostic et de Recherche sur le SIDA et Autres Maladies Infectieuses, CHU de Treichville, Abidjan, Côte d’Ivoire and Département de Biochimie et Biologie Moléculaire, UFR Sciences Pharmaceutiques et Biologiques, Université Félix Houphouet – Boigny, Côte d’Ivoire.
J. A. Sibli Koffi
Département de Biochimie et Biologie Moléculaire, UFR Sciences Pharmaceutiques et Biologiques, Université Félix Houphouet – Boigny, Côte d’Ivoire.
F. Koné
CeDReS Teaching Hospital of Treichville, BP V 3, Abidjan, Côte d’Ivoire.
H. Touré
Centre National de Transfusion Sanguine, Abidjan, Côte d’Ivoire.
M. Traoré
Centre Anti-tuberculeux de Treichville Abidjan, 05 BP 1553 Abj 05, Abidjan, Côte d’Ivoire.
K. S. M. Domoua
Service de Pneumo-Phtisiologie, CHU de Treichville, Abidjan, Côte d’Ivoire.
F. H. Yapi
Laboratoire de Biochimie, Pharmacodynamie, UFR Bosciences, Université Félix Houphouet- Boigny, Côte d’Ivoire.
D. Monnet
Département de Biochimie et Biologie Moléculaire, UFR Sciences Pharmaceutiques et Biologiques, Université Félix Houphouet – Boigny, Côte d’Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Introduction: The antioxidant property of haptoglobin (Hp), variable according to the phenotypes, is associated with the pathogenesis of various diseases. Phenotype of haptoglobin is associated with iron metabolism, which is essential for the growth of Mycobacterium tuberculosis. A potential link between Hp phenotype and blood soluble transferrin receptor (sTfR) has not yet been established. This study aimed to determine the association between Hp phenotype and blood sTfR during tuberculosis (TB).
Materials and Methods: With a case-control study involving 70 TB patients and 70 healthy subjects were performed phenotyping of Hp by electrophoresis in polyacrylamide gel and determination of blood sTfR by immunoturbidimetry.
Results: The 3 major phenotypes of Hp were found: Hp1-1, Hp2-1, Hp2-2. Hp2-2 phenotype was less common among TB patients (17.4% vs 82.6%, p < 0.001). The average rate of TB sTfR was lower than that of healthy subjects (27.5% vs 5.7%, p = 0,014). In this group, blood sTfR of Hp2 subjects (Hp2-1 and Hp2-2) was lower than those of Hp1 subjects (p = 0.011). However, in healthy subjects, it appeared no association between Hp phenotype and blood sTfR.
Conclusion: the percentage of Hp2-2 was lower in patients with tuberculosis. Furthermore, Hp1-1 phenotype and Hp1 genotype were the most frequent among tuberculosis. In spite of M. tuberculosisinfection, iron-dependent, no association was found between Hp and iron deficiency.
Keywords: Phenotype, haptoglobin, soluble transferrin receptor;, Mycobacterium tuberculosis, tuberculosis.