Association of MMP 9-1562 C/T Single Nucleotide Polymorphism with the Susceptibility to Lung Cancer Disease in South Iranian Population
Mohammad Jafari
Cellular and Molecular Gerash Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Aliyar Pirouzi
Cellular and Molecular Gerash Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Mehdi Mohsenzadeh
Cellular and Molecular Gerash Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Ali Asghar Kusari
Cellular and Molecular Gerash Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Samaneh Hajihosseini *
Genetic Division, Department of Biology, Faculty of Sciences, Isfahan University, Isfahan, I.R, Iran.
*Author to whom correspondence should be addressed.
Abstract
Aims: The aim of this study was to investigate the association of MMP9 -1562 C /T single nucleotide polymorphism with lung cancer in south Iranian population. Matrix metalloproteinases (MMPs) are a family of highly conserved metal-dependent proteolytic enzymes that are able to degrade ECM components and regulate various cell behaviors. Among several candidate genes, MMP9 is one of the most important genes known to play a key role in relation to lung cancer initiation and progression. A common -1562(C/T) single nucleotide polymorphism in the promoter region of MMP9 was reported to have an association with lung cancer disease.
Study Design: A case - control study was carried out using 90 lung cancer patients and 100 healthy controls.
Place and Duration of Study: Cellular and Molecular Gerash Research Center, Shiraz University of Medical sciences, shiraz, Iran, between 2010-2012.
Methodology: The association of MMP9 promoter single nucleotide polymorphism and lung cancer was examined in a PCR-RFLP method. Our results suggest an association between MMP9 single nucleotide polymorphism and lung cancer initiation and progression.
Conclusion: According to our results, the frequency of T allele in patient group (OR=4.6111, 95% CI, 1.9005 to 11.1879) and TT genotype in smoker group (OR=1.7726, 95% CI, 0.7947 to 3.9538) is higher compared to other groups.
Keywords: Matrix metalloproteinase, single nucleotide polymorphism, PCR-RFLP, lung cancer.