Vitamin E Supplementation Improves Oxidant-antioxidant Balance in Chronic Renal Failure Patients Treated by Hemodialysis
Amina Tiali
Laboratory of Clinical Nutrition and Metabolic, Department of Biology. Faculty of Nature and Life. University of Oran Es-Senia, 31100, Algeria.
Ouarda Taleb-Belkadi
Laboratory of Clinical Nutrition and Metabolic, Department of Biology. Faculty of Nature and Life. University of Oran Es-Senia, 31100, Algeria.
Hadja Fatima Tbahriti
Laboratory of Clinical Nutrition and Metabolic, Department of Biology. Faculty of Nature and Life. University of Oran Es-Senia, 31100, Algeria.
Nassima Bouzidi-Bekada
Laboratory of Clinical Nutrition and Metabolic, Department of Biology. Faculty of Nature and Life. University of Oran Es-Senia, 31100, Algeria.
Abbou Kaddous
Department of Nephrology, University Hospital Establishment of Oran, Algeria.
Bouchenak Malika
Laboratory of Clinical Nutrition and Metabolic, Department of Biology. Faculty of Nature and Life. University of Oran Es-Senia, 31100, Algeria.
Khedidja Mekki *
Laboratory of Clinical Nutrition and Metabolic, Department of Biology. Faculty of Nature and Life. University of Oran Es-Senia, 31100, Algeria.
*Author to whom correspondence should be addressed.
Abstract
Aims: The purpose of this study was to evaluate the effect of vitamin E supplementation on oxidant-antioxidant balance in chronic renal failure patients treated by hemodialysis.
Study Design: The study utilized a randomized experimental design. The experimental intervention consisted of vitamin E supplementation
Place and Duration of Study: 40 patients on hemodialysis (M/W=22/18; 36±12 years) received nutritional councils based on the NKF K/DOQI (National Kidney Foundation-Kidney Disease Outcomes Quality Initiative) guidelines. Patients were randomized into 2 groups:one was used as control and the second group was treated by vitamin E supplementation (100mg/day=146IU/d) during 30 days.
Methodology: Pro-oxidant status was assessed by thiobarbituric acid reactive substances, hydroperoxides and carbonyls analysis. Antioxidant defence was performed by the analysis of Superoxide dismutase, Catalase, Glutathione reductase activities and Vitamin E amounts.
Results: At 30 days of supplementation, in treated patients compared to controls, levels of triacylglycerols and total cholesterol were unchanged. Hydroperoxides concentrations were decreased (p<0.001) while thiobarbituric acid reactive substances concentrations were unchanged. Carbonyls levels were decreased (p<0.001). High concentrations of vitamin E were noted in treated group (p<0.01). Similar superoxide dismutase activity was noted. However, an increase in vitamin E concentrations, catalase and glutathione reductase activities were observed in treated group (p<0.01).
Conclusion: In conclusion, in hemodialysis patients, vitamin E supplementation was without effect on lipid profile. However, vitamin E exerts a protective effect on cardiovascular diseases by decreasing radical attack of biological molecules and increasing antioxidant defense.
Keywords: Pro-oxidant, antioxidant defence, nutritional intervention, Vit E supplementation, hemodialysis