Evaluation of IL-10 and IL-12B Gene Polymorphisms on the Response to the Standard of Care Therapy in Chronic Hepatitis C Patients: An Egyptian Cohort Study
Eman Medhat
Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University, Egypt.
Hosny Salama
Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University, Egypt.
Hanan Fouad
Medical Biochemistry, Faculty of Medicine, Cairo University, Egypt.
Raghda Marzaban *
Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University, Egypt.
Naglaa Zayed
Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University, Egypt.
Dalia Omran
Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University, Egypt.
Zeinab Zakaria
Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University, Egypt.
Tamer Elbaz
Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University, Egypt.
Ahmed Ramadan
Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Background: Interleukin-10 (IL-10) and IL–12B single nucleotide polymorphisms (SNPs) are confirmed to influence the natural history of hepatitis C virus (HCV) infection, and the response to treatment. This work aimed at evaluating the impact of SNPs in IL-10 gene at positions _1082, _819, and_592 and IL-12B gene on the response to the standard of care (SOC) treatment in Egyptian patients with chronic HCV.
Methods: Eighty seven patients with chronic HCV treated by SOC therapy and 20 healthy controls were tested for SNPs in IL-10 at _1082 G/A, _819 C/T and_592 C/A and in IL-12B (30-UTR 1188-A/C) by polymerase chain reaction (PCR). Patients were divided according to their virologic response into 2 groups; group Ι=patients who achieved sustained virologic response (SVR) and group Π = non responder (NR) patients.
Results: SNPs of IL-10 at _1082 G/A and_819 C/T showed that; GA and TT genotypes were significantly related to SVR (P=0.001 and 0.007 respectively). IL-12 gene polymorphisms showed that; CC genotype was significantly related to SVR group (P=0.01) while AA genotype was significantly related to NR (P=0.01).
Conclusions: Studying SNPs of IL-10_1082 G/A, IL-10_819 C/T and IL-12B (30-UTR 1188-C/A) proved GA, TT and CC genotypes, respectively, to be good predictors for SVR. Conversely, SNPs of IL-12 C/A proved AA genotype to be good predictor for NR.
Keywords: Hepatitis C virus (HCV), sustained virologic response (SVR), non responder (NR), standard of care (SOC), single nucleotide polymorphism (SNP)