Evaluation of Molecular Detection for Viral Hemorrhagic Infection Cases of Yellow Fever and Dengue Fever in Côte d’Ivoire 2010-2012, West Africa
Ngazoa Kakou E. Solange *
Department of Technics and Technologies, Plateform of Molecular Biology, Pasteur institute, Abidjan BP 490 Abidjan 01, Côte d’Ivoire
Niedrig Mattias
Robert Koch Institute, Center for Biological Security, Nordufer 20, Berlin 13353, Germany
Akran Veronique
Department of Technics and Technologies, Plateform of Molecular Biology, Pasteur institute, Abidjan BP 490 Abidjan 01, Côte d’Ivoire
Aoussi Serge
Department of Technics and Technologies, Plateform of Molecular Biology, Pasteur institute, Abidjan BP 490 Abidjan 01, Côte d’Ivoire
Sylla Aboubacar
Department of Technics and Technologies, Plateform of Molecular Biology, Pasteur institute, Abidjan BP 490 Abidjan 01, Côte d’Ivoire
Yavo Albert
Department of Technics and Technologies, Plateform of Molecular Biology, Pasteur institute, Abidjan BP 490 Abidjan 01, Côte d’Ivoire
Sangaré Flany
Department of Technics and Technologies, Plateform of Molecular Biology, Pasteur institute, Abidjan BP 490 Abidjan 01, Côte d’Ivoire
Dosso Mireille
Department of Technics and Technologies, Plateform of Molecular Biology, Pasteur institute, Abidjan BP 490 Abidjan 01, Côte d’Ivoire
*Author to whom correspondence should be addressed.
Abstract
Background: Viral hemorrhagic fevers are emergent and endemic in Africa and in South America. In Côte d’Ivoire, Yellow fever cases were reported yearly and the distribution of mosquitoes in the country are the main factors for high incidence of Flaviviruses. The poorly reporting of viral hemorrhagic fever cases in some regions, the lack of international interest land and the underestimation of molecular surveillance method contribute to increase the risk for public health.
Objectives: To evaluate the performance of molecular diagnostic methods in national surveillance of two emergent Flaviviruses, Yellow fever virus and Dengue virus in Côte d’Ivoire.
Study Design: 63 sera from suspected cases in 2010-2011 of viral hemorrhagic fevers were analyzed to detect viral RNA of Flaviviruses and to compare the results in three different methods.
Results and Conclusion: The Flavivirus RT-PCR has showed the high molecular detection by12% and 6% for real time PCR. The methods are specific and high sensitive for the screening of tick and mosquito-borne Flaviviruses in clinical samples. This study confirms the high circulation of Flaviviruses and the introduction of Dengue virus in Côte d’Ivoire. The combination of real time PCR and the Flavivirus RT-PCR contribute to ameliorate the detection panel of molecular detection in Côte d’Ivoire and was a suitable method for the National Laboratory Reference.
Keywords: Hemorrhagic fever, flaviviruses, molecular detection, RT-PCR, dengue, yellow fever, West Africa