Main Article Content
Aim: The aim of this study is to determine the quality of our tissue processing through participation a multicentre research programme as part of external quality control.
Study Design: A analytical retrospective study.
Place and Duration of Study: Department of Pathology University of Calabar Teaching Hospital, July 2019.
Methodology: An analytical study reviewing the performance of lymphoma tissue contributed to a Sub-Saharan African Lymphoma consortium study commissioned in 2008/2009 for which results were published in 2012. Twelve formalin fixed paraffin embedded lymphoma tissue were tested with a panel of 40 immunohistochemistry antibodies. The tissues were cut into 480 cores placed on slides before the test.
Results: The tissues were from 5 women and 7 men. The mean age was 37years, median age 45 years and modal age was 60 years. Twenty six percent of the sectioned cores lifted at test and could not therefore produce results. The reason for the lift off was tissue brittleness. Seventy four percent (74%) had intact cores on slides and produced a staining reaction although fragile antibodies like Ki 67 and bcl6 produced non reliable results while hardy antibodies like CD20 were more reliable.
Conclusion: The quality of histopathology biopsy results in the Department of Pathology University of Calabar teaching hospital needs to be improved. The strategies to achieve this involves the institution of continuous quality control and quality assurance.
Namiki T. [Quality assurance of pathology laboratory, standardization of surgical pathology r eport and consultation: recent trend]. Rinsho Byori. 1994;42(9): 897-901.
Parvin CA, Baumann NA. Assessing Quality Control Strategies for HbA1c Measurements From a Patient Risk Perspective. J Diabetes Sci Technol. 2018; 12(4):786-91.
Rao S, Masilamani S, Sundaram S, Duvuru P, Swaminathan R. Quality Measures in Pre-Analytical Phase of Tissue Processing: Understanding Its Value in Histopathology. J Clin Diagn Res. 2016;10(1):EC07-11.
Errors in a Stat Laboratory: Types and Frequencies 10 Years Later Clinical Chemistry. 2007;53(7):1338–42.
Robert Hawkins MD. Managing the Pre- and Post-analytical Phases of the Total Testing Process. Ann Lab Med. 2012;32:5-16
Muhammad Ashraf Sharif SM, Nadira Mamoon 3, Shahid Jamal 4, Muhammad Luqman 5. Clinician’s responsibility in pre-analytical quality assurance of histopathology. Pak J Med Sci (Part-I). 2007;23(5):720-3.
Zubair Ahmad RI, Nasir Uddin, Arsalan Ahmed, Saira Fatima. Errors in Surgical Pathology Reports: A Study from a Major Center in Pakistan Asian Pac J Cancer Prev. 2016;17(4):1869-74.
Cardoso Filho LI, Tavares SBN, Siqueira MLB, Dos Passos EN, Araujo N, Martins JMA, et al. Internal quality control indicators in cervical cytopathology of a university laboratory. Cytopathology. 2018; 29(4):355-60.
Ibrahim M Zardawi GB, Sanjiv Jain, Michael Brown. Internal quality assurance activities of a surgical pathology department in an Australian teaching hospital. J Clin Pathol. 1998;51:695-9.
Dr. Aarti B, Bhattacharya DSB, Dr. Rumpa Das , Dr. Priyankar Sharma, Dr. Shashwat Vidyadhar. An audit of diagnostic surgical pathology: A tool for quality assurance. Original study in a rural teaching hospital in Eastern Uttar Pradesh, India IOSR Journal of Dental and Medical Sciences (IOSR-JDMS). 2015;14(5) Ver. III):32-3.
Roque R, Henrique H, Aguiar P. Preanalytic errors in anatomic pathology: Study of 10,574 cases from five Portuguese hospitals. Diagnosis (Berl). 2015;2(3):181-8.
Fleming KA, Naidoo M, Wilson M, Flanigan J, Horton S, Kuti M, et al. High-Quality Diagnosis: An Essential Pathology Package. In: Rd, Jamison DT, Gelband H, Horton S, Jha P, Laxminarayan R, et al., editors. Disease Control Priorities: Improving Health and Reducing Poverty. Washington (DC); 2017.
Ferreira MFSLCL. Diagnostic errors in surgical pathology. J Bras Patol Med Lab. 2017;53(2):124-9.
Kim SW, Roh J, Park CS. Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips. J Pathol Transl Med. 2016;50(6):411-8.
Gown AM. Diagnostic Immunohistochemistry: What Can Go Wrong and How to Prevent It. Arch Pathol Lab Med. 2016;140(9):893-8.
Pathology AoDoAaS. Recommendations for Quality Assurance and Improvement in Surgical and Autopsy Pathology. Am J Clin Pathol 2006;126:337-40.
Rickert RR. Quality assurance in anatomic pathology. Clin Lab Med. 1986;6(4):697-706.
Cowan DF. Quality assurance in anatomic pathology. An information system approach. Arch Pathol Lab Med. 1990; 114(2):129-34.
Collina G, Caprara G, Di Tommaso L. [Quality control in pathological anatomy: 10 years' experience]. Pathologica. 2003; 95(4):171-8.
Zarbo RJ. Quality assessment in anatomic pathology in the cost-conscious era. Am J Clin Pathol. 1996;106(4Suppl1):S3-10.
Pai S, Frater JL. Quality management and accreditation in laboratory hematology: Perspectives from India. Int J Lab Hematol. 2019;41 (Suppl 1):177-83.
Kraemer BB. Quality assurance activities of the College of American Pathologists. Acta Cytol. 1989;33(4):434-8.
Bachner P, Howanitz PJ, Lent RW. Quality improvement practices in clinical and anatomic pathology services. A College of American Pathologists Q-probes study of the program characteristics and performance in 580 institutions. Am J Clin Pathol. 1994;102(5):567-71.